蛋白抗体
分子生物学
检测试剂盒
基因
辅助试剂
规格：
查看说明书| Product Name: | His Tag Rabbit pAb |
| Cat No.: | DPA05256 |
| Clonality: | Polyclonal |
| Species Reactivity: | Transfected |
| Tested Applications: | WB,IHC,ICC/IF,FC,IP |
| Recommended Dilution: | WB: 1:1000 IHC: 1:500 ICC/IF: 1:20 FC: 1:500 IP: 1:20 |
| Size: | 30ul 50ul 100uL |
| Format: | Liquid |
| Source: | Rabbit |
| Purification Method: | Affinity Purification |
| Isotype: | IgG |
| Conjugate: | Un-conjugated |
| Storage: | Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide a |
| Immunogen: | A synthetic peptide of His tag |
| Calculated Molecular Weight: | 1 kDa |
| Observed Molecular Weight: | According to the fused protein |
| Background: | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. |
| Category: | Primary Ab |
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