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His Tag Rabbit pAb

Cat No.: DPA05257
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Product Name: His Tag Rabbit pAb
Cat No.: DPA05257
Clonality: Polyclonal
Species Reactivity: Transfected
Tested Applications: WB,IHC,FC,IP
Recommended Dilution: WB: 1:1000
IHC: 1:500
FC: 1:500
IP: 1:20
Size: 30ul 50ul 100uL
Format: Liquid
Source: Rabbit
Purification Method: Affinity Purification
Isotype: IgG
Conjugate: Un-conjugated
Storage: Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide a
Immunogen: A synthetic peptide of His tag
Calculated Molecular Weight: 1 kDa
Observed Molecular Weight: According to the fused protein
Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Category: Primary Ab
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