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Renilla Luciferase Rabbit pAb

Cat No.: DPA05273
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Product Name: Renilla Luciferase Rabbit pAb
Cat No.: DPA05273
Clonality: Polyclonal
Species Reactivity: Other
Tested Applications: WB,IHC,ICC/IF,FC
Recommended Dilution: WB: 1:1000
ICC/IF: 1:200
Size: 30ul 50ul 100uL
Format: Liquid
Source: Rabbit
Purification Method: Affinity Purification
Isotype: IgG
Conjugate: Un-conjugated
Storage: Store at -20°C. Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide a
Immunogen: A synthetic peptide of Renilla Luciferase
Calculated Molecular Weight: 36 kDa
Observed Molecular Weight: According to the fused protein
GenBank Accession Number: P27652
Synonyms: Renilla Luciferase
Background: Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Category: Primary Ab
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